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KMID : 0377619720230030319
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Korean Jungang Medical Journal
1972 Volume.23 No. 3 p.319 ~ p.325
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Effects of Mercuric Chloride and Carbon Tetrachloride on Mast Cells in the Mesentery of Mice
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Abstract
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This experiment was performed in an attempt to study the effects of mercuric chloride and carbon tetrachloride, which are poisonous to the kidney and liver respectively, on the mast cells in the mesenteries of mice.
Healthy adult male mice weighing about 20g were divided into three groups; normal, mercuric chloride injected, and carbon tetrachloride injected. Animals which were injected intravenously with a single does of 15mg ´of mercuric chloride per kg body weight were sacrificed on the 12th. 18th, 24th, 30th, and 48th hour after the injection , and the ones which were subcutaneously injected with a single does of 4.0cc of carbon tetrachloride per kg body weight were killed on the 1st, 2nd, 3rd, 5th, and 7th day after the injection.
Mesenteries were made in toto preparation, fixed in 10% neutral formalin solution, and stained with Pugh´s solution. The average numbers of mast cells were counted in 50 fields under 160 magnification, and morphological changes of the cells, ,granularity and stain reaction of the specific granules were also observed.
The results were as follows:
Injection of mercuric chloride or carbon tetrachloride did not show significant changes in the number of mast cells, but caused irregularities of form, distinct degranulation, decrease of stain reaction or insatiability in many cells. It is presumed that this phenomenon was caused by inhibitory actions of these poisons to the intracellular enzymes in the mast cell.
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